Applications of bromelain from pineapple waste towards acne

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Isolation and Purification of Bromelain from Waste Peel of Pineapple for Therapeutic Application

The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, mainta...

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Thermal stability of free bromelain and bromelain-polyphenol complex in pineapple juice

Pineapple (Ananas comosus), Bromeliaceae family, is a fruit grows in tropical countries including Malaysia. This fruit has several pharmacological benefits due to the presence of high concentration of bromelain (cysteine proteases). Condition of elevated temperature will induce deformation of enzyme and result in loss of activity. Sulfhydryl groups in cysteine proteases are readily to be oxidiz...

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Application of bromelain powder produced from pineapple crowns in tenderising beef round cuts

The purpose of this study is to determine the effect of bromelain produced from pineapple crowns on the quality of beef round cuts. The beef was treated with bromelain at the following condition; the pH of beef was 5.6, the immersion temperature was 60oC, the concentration of bromelain solution was 0.17% and the immersion time was 10 minutes. Bromelain decreased the hardness, water holding capa...

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Bromelain, from pineapple stems, proteolytically blocks activation of extracellular regulated kinase-2 in T cells.

Recently, it has emerged that extracellular proteases have specific regulatory roles in modulating immune responses. Proteases may act as signaling molecules to activate the Raf-1/extracellular regulated kinase (ERK)-2 pathway to participate in mitogenesis, apoptosis, and cytokine production. Most reports on the role of protease-mediated cell signaling, however, focus on their stimulatory effec...

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Partitioning of bromelain from pineapple peel (Nang Lae cultv.) by aqueous two phase system

Pineapple peel is a potential source for the extraction of beneficial bioactive compounds due to the large amount of waste after processing. The extraction of bromelain from pineapple peel by using aqueous two-phase system (ATPs) and its application in muscle models were investigated. Bromelain predominantly partitions to the polyethylene glycol (PEG) rich top phase. The highest enzyme activity...

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ژورنال

عنوان ژورنال: Saudi Journal of Biological Sciences

سال: 2021

ISSN: 1319-562X

DOI: 10.1016/j.sjbs.2020.11.032